一个抗病性增强的水稻类病变突变体的蛋白质组学研究

doi:10.3969/j.issn.1001-7216.2014.06. 001

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (6): 559-569.DOI: 10.3969/j.issn.1001-7216.2014.06. 001

• 研究报告 • 下一篇

一个抗病性增强的水稻类病变突变体的蛋白质组学研究

韩雪颖^1,2 ,杨勇² ,余初浪² ,张文浩³ ,叶胜海² ,陈斌² ,程晨² ,程晔² ,严成其² ,陈剑平^1,2,*

¹南京农业大学植物保护学院，南京 210095；²浙江省植物有害生物防控重点实验室——省部共建国家重点实验室培育基地/农业部植保生物技术重点实验室/浙江省植物病毒学重点实验室/浙江省农业科学院病毒学与生物技术研究所，杭州 310021；³中国计量学院生命科学学院，杭州310018；

收稿日期:2014-04-16 修回日期:2014-06-27 出版日期:2014-11-10 发布日期:2014-11-10
通讯作者: 陈剑平1,2,*
基金资助:
国家973计划资助项目(2012CB722504)；国家自然科学基金青年项目(31101208, 31201482)；浙江省自然科学基金重点项目(Z14C140001)。

A Proteomic Study on a DiseaseResistanceEnhanced Rice Lesion Mimic Mutant

HAN Xueying^1,2, YANG Yong², YU Chulang², ZHANG Wenhao³ , YE Shenghai², CHEN Bin², CHENG Chen²,CHENG Ye², YAN Chengqi², CHEN Jianping ^1,2,*

¹College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; ²State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Biotechnology in Plant Protection, MOA, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China; ³College of Life Science, China Jiliang University, Hangzhou 310018, China;

Received:2014-04-16 Revised:2014-06-27 Online:2014-11-10 Published:2014-11-10
Contact: CHEN Jianping1,2,*

摘要/Abstract

摘要： 水稻类病变突变体chl1具有抗病性增强的类病变表型，对水稻白叶枯病和稻瘟病都具有很强的抗性。利用蛋白质组学技术分析chl1与其野生型之间的差异表达蛋白，探讨chl1类病变表型的形成和抗病反应的分子机制。利用荧光双向差异凝胶电泳(twodimensional fluorescence difference gel electrophoresis, 2DDIGE)技术和质谱分析，chl1中共鉴定到70个差异表达的蛋白点，包括46个上调蛋白点和24个下调蛋白点。这些蛋白点参与不同的生物过程，包括防御相关、光合作用、氧化还原、氨基酸/蛋白质代谢、分子伴侣、碳水化合物代谢。对这些差异表达蛋白进行生物信息分析，推测它们所在的复杂调控网络可能参与chl1叶片细胞程序性死亡(programmed cell death, PCD)及其抗病性的调控。

关键词: 水稻, 类病变突变体, 细胞程序性死亡, 抗病性, 荧光双向差异凝胶电脉

Abstract: A lesionmimic mutant in rice (Oryza sativa L.), chloroplasticH2O2induced lesion 1 (chl1), has enhanced resistance to rice blast and bacterial blight. To understand the molecular mechanisms underlying this phenotype and its resistance responses, a proteomicsbased approach was used to identify differentiallyexpressed proteins between chl1 and its wild type. Using twodimensional fluorescence difference gel electrophoresis technology and mass spectrometry, 70 protein spots were successfully identified, of which 46 were upregulated and 24 were downregulated in the mutant. These differentiallyexpressed proteins are involved in diverse biological processes including disease resistance, photosynthesis, oxidationreduction reaction, amino acid/protein metabolism, chaperoning and carbohydrate metabolism. The complex regulatory network in which these proteins are involved may play an important role in regulating the programmed cell death and the resistance reaction in chl1.

Key words: rice, lesionmimic mutant, programmed cell death, disease resistance, 2DDIGE

中图分类号:

韩雪颖1,2 ,杨勇2 ,余初浪2 ,张文浩3 ,叶胜海2 ,陈斌2 ,程晨2 ,程晔2 ,严成其2 ,陈剑平1,2,*. 一个抗病性增强的水稻类病变突变体的蛋白质组学研究[J]. 中国水稻科学, 2014, 28(6): 559-569.

HAN Xueying1,2, YANG Yong2, YU Chulang2, ZHANG Wenhao3 , YE Shenghai2, CHEN Bin2, CHENG Chen2,CHENG Ye2, YAN Chengqi2, CHEN Jianping1,2,*. A Proteomic Study on a DiseaseResistanceEnhanced Rice Lesion Mimic Mutant[J]. Chinese Journal of Rice Science, 2014, 28(6): 559-569.

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一个抗病性增强的水稻类病变突变体的蛋白质组学研究

A Proteomic Study on a DiseaseResistanceEnhanced Rice Lesion Mimic Mutant

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